A cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causes growth anomalies by disrupting brassinosteroid signaling

Description:

Article on a cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causing growth anomalies by disrupting brassinosteroid signaling.

Creator(s):
Creation Date: April 15, 2011
Partner(s):
UNT College of Arts and Sciences
Collection(s):
UNT Scholarly Works
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Total Uses: 115
Past 30 days: 7
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Creator (Author):
Dasgupta, Kasturi

University of North Texas

Creator (Author):
Ganesan, Savita

Amyris Biotechnologies, Inc.

Creator (Author):
Manivasagam, Sindhu

University of North Texas

Creator (Author):
Ayre, Brian G.

University of North Texas

Publisher Info:
Publisher Name: BioMed Central Ltd.
Place of Publication: [London, United Kingdom]
Date(s):
  • Creation: April 15, 2011
Description:

Article on a cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causing growth anomalies by disrupting brassinosteroid signaling.

Degree:
Department: Biological Sciences
Note:

Abstract: Background: Cytochrome P450 monooxygenases form a large superfamily of enzymes that catalyze diverse reactions. The P450su1 gene from the soil bacteria Streptomyces griseolus encodes CYP105A1 which acts on various substrates including sulfonylurea herbicides, vitamin D, coumarins, and based on the work presented here, brassinosteroids. P450su1 is used as a negative-selection marker in plants because CYP105A1 converts the relatively benign sulfonyl urea pro-herbicide R7402 into a highly phytotoxic product. Consistent with its use for negative selection, transgenic Arabidopsis plants were generated with P450su1 situated between recognition sequences for FLP recombinase from yeast to select for recombinase-mediated excision. However, unexpected and prominent developmental aberrations resembling those described for mutants defective in brassinosteroid signaling were observed in many of the lines. Results: The phenotypes of the most affected lines included severe stunting, leaf curling, darkened leaves characteristic of anthocyanin accumulation, delayed transition to flowering, low pollen and seed yields, and delayed senescence. Phenotype severity correlated with P450su1 transcript abundance, but not with transcript abundance of other experimental genes, strongly implicating CYP105A1 as responsible for the defects. Germination and seedling growth of transgenic and control lines in the presence and absence of 24-epibrassinolide indicated that CYP105A1 disrupts brassinosteroid signaling, most likely by inactivating brassinosteroids. Conclusions: Despite prior use of this gene as a genetic tool, deleterious growth in the absence of R7402 has not been elaborated. The authors show that this gene can cause aberrant growth by disrupting brassinosteroid signaling and affecting homeostasis.

Physical Description:

13 p. : col. ill.

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Subject(s):
Keyword(s): P450 | genes | plants | brassinosteroid signaling | homeostasis
Source: BMC Plant Biology, 2011, London: BioMed Central Ltd.
Partner:
UNT College of Arts and Sciences
Collection:
UNT Scholarly Works
Identifier:
  • ARK: ark:/67531/metadc81383
Resource Type: Article
Format: Text
Rights:
Access: Public
Citation:
Publication Title: BMC Plant Biology
Volume: 11
Issue: 67
Pages: 13
Peer Reviewed: Yes