Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene

Description:

This article discusses the effect and overall importance of the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene on carbon partitioning and plant growth.

Creator(s):
Creation Date: January 20, 2009
Partner(s):
UNT College of Arts and Sciences
Collection(s):
UNT Scholarly Works
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Total Uses: 157
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Creator (Author):
Srivastava, Avinash C.

University of North Texas; Samuel Roberts Noble Foundation

Creator (Author):
Ganesan, Savita

University of North Texas; Amyris Biotechnologies, Inc.

Creator (Author):
Ismail, Ihab O.

University of North Texas

Creator (Author):
Ayre, Brian G.

University of North Texas

Publisher Info:
Publisher Name: BioMed Central Ltd.
Place of Publication: [London, United Kingdom]
Date(s):
  • Creation: January 20, 2009
Description:

This article discusses the effect and overall importance of the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene on carbon partitioning and plant growth.

Degree:
Department: Biological Sciences
Note:

Abstract: Background: AtSUC2 (At1g22710) from Arabidopsis thaliana encodes a phloem-localized sucrose/proton symporter required for efficient photoassimilate transport from source tissues to sink tissues. AtSUC2 plays a key role in coordinating the demands of sink tissues with the output capacity of source leaves, and in maintaining phloem hydrostatic pressure during changes in plant-water balance. Expression and activity are regulated, both positively and negatively, by developmental (sink or source transition) and environmental cues, including light, diurnal changes, photoassimilate levels, turgor pressure, drought and osmotic stress, and hormones. Results: To assess the importance of this regulation to whole-plant growth and carbon partitioning, AtSUC2 cDNA was expressed from two exotic, phloem-specific promoters in a mutant background debilitated for AtSUC2 function. The first was a promoter element from Commelina Yellow Mottle Virus (CoYMV), and the second was the rolc promoter from Agrobacterium rhizogenes. CoYMVp::AtSUC2 cDNA restored growth and carbon partitioning to near wild-type levels, whereas plants harboring rolCp::AtSUC2 cDNA showed only partial complementation. Conclusion: Expressing AtSUC2 cDNA from exotic, phloem-specific promoters argues that strong, phloem-localized expression is sufficient for efficient transport. Expressing AtSUC2 from promoters that foster efficient phloem transport but are subject to regulatory cascades different from the endogenous sucrose/proton symporter genes has implications for biotechnology.

Physical Description:

13 p.

Language(s):
Subject(s):
Keyword(s): carbon partitioning | sucrose-proton symporter | genes
Source: BMC Plant Biology, 2009, London: BioMed Central Ltd.
Partner:
UNT College of Arts and Sciences
Collection:
UNT Scholarly Works
Identifier:
  • DOI: 10.1186/1471-2229-9-7 |
  • ARK: ark:/67531/metadc78288
Resource Type: Article
Format: Text
Rights:
Access: Public
Citation:
Publication Title: BMC Plant Biology
Volume: 9
Issue: 7
Pages: 13
Peer Reviewed: Yes