Genome-wide mutagenesis of Zea mays L. using RescueMu transposons

Description:

This article discusses genome-wide mutagenesis of Zea mays L. using RescueMu transposons.

Creator(s):
Creation Date: 2004  
Partner(s):
UNT College of Arts and Sciences
Collection(s):
UNT Scholarly Works
Usage:
Total Uses: 64
Past 30 days: 7
Yesterday: 2
Creator (Author):
Fernandes, John

Stanford University

Creator (Author):
Dong, Qunfeng

University of North Texas; Iowa State University

Creator (Author):
Schneider, Bret

Stanford University

Creator (Author):
Morrow, Darren J.

Stanford University

Creator (Author):
Nan, Guo-Ling

Stanford University

Creator (Author):
Brendel, Volker

Iowa State University

Creator (Author):
Walbot, Virginia

Stanford University

Publisher Info:
Publisher Name: BioMed Central Ltd.
Place of Publication: [London, United Kingdom]
Date(s):
  • Creation: 2004
Description:

This article discusses genome-wide mutagenesis of Zea mays L. using RescueMu transposons.

Degree:
Department: Biological Sciences
Note:

Abstract: Derived from the maize Mu I transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.

Physical Description:

20 p.

Language(s):
Subject(s):
Keyword(s): maize gene discoveries | mutant phenotypic analysis | RescueMu
Source: Genome Biology, 2004, London: BioMed Central Ltd.
Partner:
UNT College of Arts and Sciences
Collection:
UNT Scholarly Works
Identifier:
  • ARK: ark:/67531/metadc77168
Resource Type: Article
Format: Text
Rights:
Access: Public
Citation:
Publication Title: Genome Biology
Volume: 5
Issue: 10
Pages: 20
Peer Reviewed: Yes