N-Acylethanolamine (NAE) Profiles Change During Arabidopsis Thaliana Seed Germination and Seedling Growth Page: 30
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NAE Concentration During Seed Germination and Seedling Growth
Total NAEs
2500
2000
1500
1000
500
0b,f c,g d
a
e,h,iTime (h)
NAE profiles by species
1500
SDes
1250 0 24
1000 E 48
E96
750 - 96
El 192
500
250
0
NAEsC
zD
uJ
z
C-A
uJ
LLI
z
0J)
C-Figure 6. NAE concentration in seeds and seedlings during germination and post-
germinative growth. Total NAE content expressed as nanograms per gram FW.
Desiccated (Des) and 24 h stratified (Strat) seed samples were immediately analyzed
and others germinated in liquid MS media, following stratification in H20 at 4oC for 24 h.
Totals (A) summed from individual molecular species profiles (B). Total saturated NAE
[12:0, 14:0, 16:0, 18:0] content (C) and 18C unsaturated NAE [18:1, 18:2, 18:3] content
(D) summed from individual species profiles. Values compared by t-test. Only those
comparisons considered to be significant (p< 0.055) are listed below. Error bars
represent SD of three to sixteen replicate extractions.Total NAE values:
(a) Des vs. Strat: p< 0.00025
(b) Des vs. 24 h: p< 0.04
(c) Des vs. 48 h: p< 0.055
(d) Des vs. 96 h: p< 0.0006
(e) Des vs. 192 h: p< 0.00000075
(f) Strat vs. 24 h: p< 0.015
(g) Strat vs. 48 h: p< 0.0045151788
(h) Strat vs. 192 h: p< 0.0055
(i) 96 h vs. 192 h: p< 0.035Total unsaturated NAE values:
(a) Des vs. Strat: p< 0.00000005
(b) Des vs. 24 h: p< 0.00015
(c) Des vs. 48 h: p< 0.00015
(d) Des vs. 96 h: p< 0.0006
(e) Des vs. 192 h: p< 0.0000000015
(f) Strat vs. 24 h: p< 0.015
(g) Strat vs. 48 h: p< 0.006
(j) Strat vs. 96 h: p< 0.01
(h) Strat vs. 192 h: p< 0.0015
(i) 96 h vs. 192 h: p< 0.000330
Total saturated NAEs
600
500
400
300
200
100
0
Time (h)
Total unsaturated NAEs
2500
2000
1500 d
1000 b,f c,g
500 e,
0 T
Time (h)B
uJ
z
C-
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Wiant, William C. N-Acylethanolamine (NAE) Profiles Change During Arabidopsis Thaliana Seed Germination and Seedling Growth, thesis, August 2006; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc5333/m1/35/: accessed April 23, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .