N-Acylethanolamine (NAE) Profiles Change During Arabidopsis Thaliana Seed Germination and Seedling Growth Page: 22
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NAEs by 192 h in all treated genotypes that was observed in untreated seedlings of all
genotypes tested. Instead, the amount of total NAEs on a per individual basis dropped
from 96 to 192 h in seedlings of all treated genotypes.
Growing the FAAH mutant lines in low concentrations of ABA (0.25 pM) had little
or no effect on the total concentration of saturated NAEs (Fig. 13). Only at 192 h did
seedlings of OE 11A exhibit a substantial increase in total saturated NAE concentration
with ABA treatment, relative to other genotypes (Fig. 13B). The total saturated NAE
content on a per individual basis was unaltered by ABA treatment in all genotypes tested
at 96 h (Fig. 14B). However, at 192 h, none of the ABA-treated seedlings of any genotype
appeared to accumulate saturated NAEs in contrast to untreated seedlings, and therefore
remained an order of magnitude below the levels observed in untreated seedlings on a
per individual basis (Fig. 14A). This suggests the synthesis of saturated NAEs during
post-germinative growth may play a substantial role in the metabolic regulation of
saturated NAE species in plant tissues.
Little effect of ABA treatment was seen in unsaturated NAE concentrations at
either 96 or 192 h-old seedlings of any genotype (Fig. 13). The concentrations of total
unsaturated NAEs in ABA-treated wild-type seedlings at 96 and 192 h (Fig. 13D) were ~2
times greater than untreated wild-type seedlings (Fig. 13C), whereas the FAAH knockout
and overexpressor lines showed no substantial adjustment of their total unsaturated NAE
concentrations with ABA treatment at either time point (Compare Figs. 13C and D).
The profiles of total unsaturated NAE content per individual depended on whether
tissue was grown in the presence or absence of 0.25 pIM ABA (Fig. 14). At 96 h, treated
wild-type and KO SK095 seedlings (Fig. 14D) contained 4 and 2 times more unsaturated22
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Wiant, William C. N-Acylethanolamine (NAE) Profiles Change During Arabidopsis Thaliana Seed Germination and Seedling Growth, thesis, August 2006; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc5333/m1/27/: accessed April 19, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .