FLP-mediated conditional loss of an essential gene to facilitate complementation assays Page: 46
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35 cycles
980C for 3 minutes - Denaturation
980C for 10 seconds - Denaturation
72C for 30 seconds - Annealing
72C for 30 seconds - Extension
72C for 10 minutes - Final Extension
4C - HoldTable 10: Cycling conditions for the first and second round of PCR amplification of the aacCl
gene cassette.46
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Ganesan, Savita. FLP-mediated conditional loss of an essential gene to facilitate complementation assays, thesis, December 2007; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc5180/m1/56/: accessed April 25, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .