FLP-mediated conditional loss of an essential gene to facilitate complementation assays Page: 90
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(a)
F
F52229.LNT-1-CP1:898 > 1834
Bar coding region (X17220): 854 > 1372
F53038.LNT 1-CP1: 512 << 1366 (corrplerrentary)
F73809.LNT-2-CP4: 1 << 949 (corrplerrentary)
1 00 11000
poo0 ,1000
I IF I I IF73809.ULNT-1-CP3: 1571,, 2460
54513.LNT-8-M13F: 1368 << 2146 (corrplerrentary),1500
2000
(b)
HindII (845)
SUC2sacI (517) FRTBar KpnI (1371)
NotI (2153)
SacI (2182)
IIpART-Bar contig 1
2460 bp
Figure 35: Sequence analysis data and sequence map of the Bar gene. (a) Contig assembly of the
sequenced fragments. (b) Annotated sequence map of the pART-Bar contig assembly.90
I
I
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Ganesan, Savita. FLP-mediated conditional loss of an essential gene to facilitate complementation assays, thesis, December 2007; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc5180/m1/100/: accessed April 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .