Experiment Station Record, Volume 89, July-December, 1943 Page: 16
xiv, 885 p. ; 24 cm.View a full description of this book.
Extracted Text
The following text was automatically extracted from the image on this page using optical character recognition software:
16 EXPERIMENT STATION RECORD [Vol. 89
Bactericidal and fungicidal properties of a crystalline protein isolated
from unbleached wheat flour, L. S. STUART and T. H. HARRIS. (U. S. D. A.).
(Cereal Chem., 19 (1942), No. 2, pp. 288-300, illus. 2).-The purified, crystallized
hydrochloride of a protein separated from unbleached wheat flour has both
bactericidal and bacteriostatic activity "in vitro." No "in vivo" activity could be
demonstrated when the substance was tested in mice against pneumococci and
streptococci. In vitro activity was greatest against gram-positive organisms,
but there was also some activity against gram-negative organisms. When tested
against Saccaromyces cerevisiae.this protein brought about the death of the yeast
cells in concentrations of 0.005 mg. per cubic centimeter or higher. Similar results
were obtained with the pathogenic fungi Debaryomyces nadiformis and Endomycopsis
albicans. However, tests on mycelial fungi failed to show any, fungicidal
activity. In vitro tests failed to show any hemolytic, bacteriolytic, or yeast-cell
lytic action.
Effect on small laboratory animals of the injection of the crystalline hydrochloride
of a sulfur protein from wheat flour, E. J. COULSON, T. H. HARRIS,
and B. AXELROD. (U. S. D. A. et al.). (Cereal Chem., 19 (1942), No. 2, pp. 301307,
illus. 4).-The bactericidal protein discussed in the preceding paper was
investigated as a possible protective against disease organisms but was found
toxic to the experimental animals when injected, though harmless by mouth in
relatively large doses. It appeared that the minimum lethal dose of the original
hydrochloride for mice was about 15 mg. per kilogram ofbody weight when the
substance was injected intraperitoneally.
The synthesis of a-amino acids from substituted acetoacetic esters,
K. E. HAMLIN, JR., and W. H. HARTUNG. (Univ. Md.). (Jour. Biol. Chem., 145
(1942), No. 2, pp. 349-357, illus. 10).-The synthesis of a-amino acids through
the nitrosation of appropriately substituted acetoacetic esters followed by hydrolysis
and hydrogenation is described. Alanine, a-aminobutyric acid, norvaline,
norleucine, isoleucine, aspartic acid, glutamic acid, phenylalanine, and o-methyltyrosine
were synthesized in good yield by this method. o-Methyltyrosine was
demethylated and converted into tyrosine.
The polarographic behavior of histidine and certain other amino-acids,
E. R. ROBERTS (AbS. in Minnesota Sta. Rpt. 1942, p. 22).--Histidine, when analyzed
polarographically, exhibits three characteristic effects, two of which, under
specific conditions, are proportional to the concentration of histidine. One of
these effects persists and retains proportionality in the presence of arginine and
lysine. This "wave" may be utilized for analytical purposes. Cystine determinations
must bemade on freshly prepared solutions. Serine, tryptophan, and tyrosine
do not lend themselves to polarographic estimation.
Technics in the synthesis of porphyrindin, H. A. LErLEVIK, R. L. HOSSFELD,
H. V. LINDSTROM, R. T. ARNOLD, and R. A. GORTNER. (Minn. Expt. Sta.). (Jour.
Organic Chem., 7 (1942), No. 2, pp. 164-168, illus. 1).-Necessary precautions,
not directly specified in previous literature, have been developed and added to
earlier methods. An improvement in the yield of nitrile from acetoxime was
made possible by cold room technics and by extractions with petroleum ether instead
of recrystallization. It was found that the most difficult step (imino ether
hydrochloride) gave excellent yields when extreme care was taken to insure
anhydrous conditions. Minor modifications in the last four steps have been made
to avoid the failures resulting from insufficient practice and knowledge of procedure.
Knowledge gained from the literature and from experiment has been
incorporated into a readily workable method.
On the identity of the so called crassulacean malic acid with isocitric
acid, G. W. PUCHnER and H. B. VICKERY. (Conn. [New Haven] Expt. Sta.).
(Jour. Biol. Chem., 145 (1942), No. 2, pp. 525-532).-An examination of the lit
Upcoming Pages
Here’s what’s next.
Search Inside
This book can be searched. Note: Results may vary based on the legibility of text within the document.
Tools / Downloads
Get a copy of this page or view the extracted text.
Citing and Sharing
Basic information for referencing this web page. We also provide extended guidance on usage rights, references, copying or embedding.
Reference the current page of this Book.
U.S. Department of Agriculture. Agricultural Research Administration. Office of Experiment Stations. Experiment Station Record, Volume 89, July-December, 1943, book, 1944; Washington D.C.. (https://digital.library.unt.edu/ark:/67531/metadc5067/m1/30/: accessed April 25, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.