Experiment Station Record, Volume 92, January-June, 1945 Page: 144
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144 EXPERIMENT STATION RECORD Vol. 92
during the three periods was 12.6, 13.6, and 12.9 1. per cow. When the vitamin
production was calculated as the average daily production per cow the values for
ascorbic acid, riboflavin, and thiamine became 255.6, 18.0, and 4.0 mg., respectively,
in period 1; 259.0, 16.9, and 4.3 mg. in period 2; and 251.1, 18.8, and 4.4 mg. in
period 3. These results are interpreted to indicate that early pasture is not superior
to a properly produced, well-selected winter ration.
Effect of pasteurization on the riboflavin content of milk, A. D. HOLMES.
(Mass. Expt. Sta.). (Jour. Amer. Dietet. Assoc., 20 (1944), No. 4, pp. 226-227).Mixed
morning and evening herd milk produced under controlled feeding and management
conditions by five breeds (Ayrshire, Guernsey, Holstein, Jersey, and Milking
Shorthorn) of normal healthy cows was pasteurized by the holding and by the
flash process. Riboflavin, determined by the fluorescence method in a semidark
laboratory, averaged 1.46 mg. per liter before and 1.43, mg. per liter after pasteurization
in the spray vat equipment (24 samples); 1.52 mg. per liter before and 1.49
mg. per liter after pasteurization in the coil vat equipment (12 samples); and 1.41
mg. per liter before and 1.42 mg. per liter after pasteurization by the flash process.
These results indicate that the consumer can be assured that he will obtain essentially
as much riboflavin from recently pasteurized milk, under the conditions described,
as he would from the same raw milk just before pasteurization.
Distribution of vitamin A in tissue as visualized by fluorescence microscopy
H. POPPER (Physiol. Rez., 24 (1944), No. 2, pp. 205-224).-This review presents
a brief historical introduction, outlines the method of determining vitamin A in
tissue by fluorescence microscopy, and considers the evidence for the specificity of
vitamin A fluorescence, the stability of the fluorescence, and the influence of carrier
substances on this property. The vitamin A distribution in different organs under
normal and pathological conditions as determined by the fluorescence technic is
reviewed. The review, based on 149 literature references, is summarized as follows:
"The microscopic visualization of vitamin A by fluorescence permits the
study of its distribution in organs. In various physiologic and pathologic conditions
the distribution undergoes characteristic changes, not indicated by chemical analysis
or bio-assay. Conclusions as to the role of vitamin A in the body can be made
which are not arrived at by other methods. The histologic method, which permits
only a rough but quick estimation as to the quantity of vitamin A present, does
not substitute for the chemical analysis but supplements it. The fluorescence microscopic
demonstration of vitamin A, however, not only aids in the investigation of
the vitamin A metabolism but also presents a new histologic method for the
differentiation of lipides independent of the biologic significance of the fluorescence."
The bodily store of vitamin A as influenced by age and by food, A. B. ROHRER
and H. C. SHERMAN (Jour. Nutr., 25 (1943), No. 6, pp. 605-609).--In studies
similar to but more extensive than those reported by Kao and Sherman (E. S. R.,
85, p. 565), offspring of rat families on diets otherwise similar but furnishing,
respectively, 3, 6, and 12 International Units of vitamin A per gram of air-dry
food were killed at 30 or 60 days and their muscle and liver tissues analyzed for
vitamin A by the single feeding method of Sherman and Todhunter. "Whether
compared at the age of 30 or 60 days, the vitamin A in the liver was found to
have been decidedly influenced by the level of nutritional intake of this vitamin.
The skeletal muscles of the same animals showed differences in the same direction,
but so small as to be of doubtful statistical significance. Storage of vitamin A in
the body, as reflected by the concentration in the liver, was found to have continued
in the second age period studied when the level of nutritional intake of the vitamin
was high, but not when it was near the minimal-adequate level."
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U.S. Department of Agriculture. Agricultural Research Administration. Office of Experiment Stations. Experiment Station Record, Volume 92, January-June, 1945, book, 1947; Washington D.C.. (https://digital.library.unt.edu/ark:/67531/metadc5064/m1/157/: accessed April 19, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.