Multi-site genetic modulation of monolignol biosynthesis suggests new routes for formation of syringyl lignin and wall-bound ferulic acid in alfalfa (Medicago sativa L.)

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Article on multi-site genetic modulation of monolignol biosynthesis suggests new routes for formation of syringyl lignin and wall-bound ferulic acid in alfalfa (Medicago sativa L.).

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12 p.

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Chen, Fang; Reddy, M. S. Srinivasa; Temple, Stephen; Jackson, Lisa A.; Shadle, Gail L. & Dixon, R. A. August 30, 2006.

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Article on multi-site genetic modulation of monolignol biosynthesis suggests new routes for formation of syringyl lignin and wall-bound ferulic acid in alfalfa (Medicago sativa L.).

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12 p.

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Abstract: Genes encoding seven enzymes of the monolignol pathway were independently downregulated in alfalfa (Medicago sativa) using antisense and/or RNA interference. In each case, total flux into lignin was reduced, with the largest effects arising from the downregulation of earlier enzymes in the pathway. The downregulation of l-phenylalanine ammonia-lyase, 4-coumarate 3-hydroxylase, hydroxycinnamoyl CoA quinate/shikimate hydroxycinnamoyl transferase, ferulate 5-hydroxylase or caffeic acid 3-O-methyltransferase resulted in compositional changes in lignin and wall-bound hydroxycinnamic acids consistent with the current models of the monolignol pathway. However, downregulating caffeoyl CoA 3-O-methyltransferase neither reduced syringyl (S) lignin units nor wall-bound ferulate, inconsistent with a role for this enzyme in 3-O-methylation ofS monolignol precursors and hydroxycinnamic acids. Paradoxically, lignin composition differed in plants downregulated in either cinnamate 4-hydroxylase or phenylalanine ammonia-lyase. No changes in the levels of acylated flavonoids were observed in the various transgenic lines. The current model for monolignol and ferulate biosynthesis appears to be an over-simplification, at least in alfalfa, and additional enzymes may be needed for the 3-O-methylation reactions of S lignin and ferulate biosynthesis.

© 2006 The Authors. Journal compilation © 2006 Blackwell Publishing Ltd.

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  • The Plant Journal, 2006, Oxford: Blackwell Publishing Ltd., pp. 113-124

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  • Publication Title: The Plant Journal
  • Volume: 48
  • Issue: 1
  • Page Start: 113
  • Page End: 124
  • Pages: 12
  • Peer Reviewed: Yes

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  • August 30, 2006

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  • Jan. 22, 2015, 9:04 a.m.

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  • Nov. 6, 2023, 2:05 p.m.

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Chen, Fang; Reddy, M. S. Srinivasa; Temple, Stephen; Jackson, Lisa A.; Shadle, Gail L. & Dixon, R. A. Multi-site genetic modulation of monolignol biosynthesis suggests new routes for formation of syringyl lignin and wall-bound ferulic acid in alfalfa (Medicago sativa L.), article, August 30, 2006; [Oxford, United Kingdom]. (https://digital.library.unt.edu/ark:/67531/metadc488163/: accessed April 25, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT College of Arts and Sciences.

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