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Article on anthocyanidin reductases from Medicago truncatula and Arabidopsis thaliana.
Physical Description
12 p.
Notes
Abstract: Anthocyanidin reductase (ANR), encoded by the BANYULS gene, is a newly discovered enzyme of the flavonoid pathway involved in the biosynthesis of condensed tannins. ANR functions immediately downstream of anthocyanidin synthase to convert anthocyanidins into the corresponding 2,3-cis-flavan-3-ols. We report the biochemical properties of ANRs from the model legume Medicago truncatula (MtANR) and the model crucifer Arabidopsis thaliana (AtANR). Both enzymes have high temperature optima. MtANR uses both NADPH and NADH as reductant with slight preference for NADPH over NADH. In contrast, AtANR only uses NADPH and exhibits positive cooperativity for the co-substrate. MtANR shows preference for potential anthocyanidin substrates in the order cyanidin > pelargonidin > delphinidin, with typical Michaelis–Menten kinetics for each substrate. In contrast, AtANR exhibits the reverse preference, with substrate inhibition at high concentrations of cyanidin and pelargonidin. (+)-Catechin and (±)-dihydroquercetin inhibit AtANR but not MtANR, whereas quercetin inhibits both enzymes. Possible catalytic reaction sequences for ANRs are discussed.
Publication Title:
Archives of Biochemistry and Biophysics
Volume:
422
Issue:
1
Page Start:
91
Page End:
102
Peer Reviewed:
Yes
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Xie, De-Yu; Sharma, Shashi B. & Dixon, R. A.Anthocyanidin reductases from Medicago truncatula and Arabidopsis thaliana,
article,
February 1, 2004;
[Amsterdam, Netherlands].
(https://digital.library.unt.edu/ark:/67531/metadc488134/:
accessed March 18, 2024),
University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu;
crediting UNT College of Arts and Sciences.