MATE Transporters Facilitate Vacuolar Uptake of Epicatechin 3'-O-Glucoside for Proanthocyanidin Biosynthesis in Medicago truncatula and Arabidopsis

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Article discussing how MATE transporters facilitate vacuolar uptake of epicatechin 3'-O-glucoside for proanthocyanidin biosynthesis in Medicago truncatula and Arabidopsis.

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19 p. : col. ill.

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Zhao, Jian & Dixon, R. A. August 2009.

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  • Zhao, Jian Samuel Roberts Noble Foundation
  • Dixon, R. A. University of North Texas; Samuel Roberts Noble Foundation

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Article discussing how MATE transporters facilitate vacuolar uptake of epicatechin 3'-O-glucoside for proanthocyanidin biosynthesis in Medicago truncatula and Arabidopsis.

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19 p. : col. ill.

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Abstract: Expression of the Arabidopsis thaliana MYB transcription factor TRANSPARENT TESTA 2 (TT2) in Medicago trunculata hairy roots induces both proanthocyanidin accumulation and the ATP-dependent vacuolar/vesicular uptake of epicatechin 3′-O-glucoside; neither process is active in control roots that do, however, possess anthocyanidin 3-O-glucoside vacuolar uptake activity. A vacuolar membrane-localized multidrug and toxic compound extrusion (MATE) transporter, Medicago MATE1, was identified at the molecular level and shown to preferentially transport epicatechin 3′-O-glucoside. Genetic evidence has implicated TT12, a tonoplastic MATE transporter from Arabidopsis, in the transport of precursors for proanthocyanidin biosynthesis in the seed coat. However, although Arabidopsis TT12 facilitates the transport of cyanidin 3-O-glucoside into membrane vesicles when expressed in yeast, there is no evidence that cyanidin 3-O-glucoside is converted to proanthocyanidins after transport into the vacuole. Here, we show that Arabidopsis TT12, like Medicago MATE1, functions to transport epicatechin 3′-O-glucoside as a precursor for proanthocyanidin biosynthesis, and Medicago MATE1 complements the seed proanthocyanidin phenotype of the Arabidopsis tt12 mutant both quantitatively and qualitatively. On the basis of biochemical properties, tissue-specific expression pattern, and genetic loss-of-function analysis, we conclude that MATE1 is an essential membrane transporter for proanthocyanidin biosynthesis in the Medicago seed coat. Implications of these findings for the assembly of oligomeric proanthocyanidins are discussed.

Copyright 2009 American Society of Plant Biologists. The following article appeared in The Plant Cell, v. 21, pp. 2323-2340, http:/​/​dx.​doi.​org/​10.​1105/​tpc.​109.​067819

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  • The Plant Cell, 2009, Washington DC: American Society of Plant Biologists, pp. 2323-2340

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  • Publication Title: The Plant Cell
  • Volume: 21
  • Issue: 8
  • Page Start: 2323
  • Page End: 2340
  • Peer Reviewed: Yes

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  • August 2009

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  • Sept. 12, 2014, 9:22 a.m.

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Zhao, Jian & Dixon, R. A. MATE Transporters Facilitate Vacuolar Uptake of Epicatechin 3'-O-Glucoside for Proanthocyanidin Biosynthesis in Medicago truncatula and Arabidopsis, article, August 2009; [Washington, D.C.]. (https://digital.library.unt.edu/ark:/67531/metadc335319/: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT College of Arts and Sciences.

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