Evaluation of the Chlorophyll/Fluorescence Sensor of the YSI Multiprobe: Comparison to an Acetone Extraction Procedure

Description:

The purpose of this study was to examine the suitability of the YSI model 6600 Environmental Monitoring System (multiprobe) for long term deployment at a site in Lewisville Lake, Texas. Specifically, agreement between a laboratory extraction procedure and the multiprobe chlorophyll/fluorescence readings was examined. Preliminary studies involved determining the best method for disrupting algal cells prior to analysis and examining the precision and linearity of the acetone extraction procedure. Cell disruption by mortar and pestle grinding was preferable to bath sonication. Comparison of the chlorophyll/fluorescence readings from the multiprobe and the extraction procedure indicated that they were significantly correlated but temperature dependent.

Creator(s): Lambert, Patricia
Creation Date: May 2001
Partner(s):
UNT Libraries
Collection(s):
UNT Theses and Dissertations
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Total Uses: 697
Past 30 days: 16
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Publisher Info:
Publisher Name: University of North Texas
Place of Publication: Denton, Texas
Date(s):
  • Creation: May 2001
  • Digitized: February 28, 2006
Description:

The purpose of this study was to examine the suitability of the YSI model 6600 Environmental Monitoring System (multiprobe) for long term deployment at a site in Lewisville Lake, Texas. Specifically, agreement between a laboratory extraction procedure and the multiprobe chlorophyll/fluorescence readings was examined. Preliminary studies involved determining the best method for disrupting algal cells prior to analysis and examining the precision and linearity of the acetone extraction procedure. Cell disruption by mortar and pestle grinding was preferable to bath sonication. Comparison of the chlorophyll/fluorescence readings from the multiprobe and the extraction procedure indicated that they were significantly correlated but temperature dependent.

Degree:
Level: Master's
Language(s):
Subject(s):
Keyword(s): chlorophyll | YSI6600 | fluorescence | algae
Contributor(s):
Partner:
UNT Libraries
Collection:
UNT Theses and Dissertations
Identifier:
  • OCLC: 50405080 |
  • UNTCAT: b2410700 |
  • ARK: ark:/67531/metadc2794
Resource Type: Thesis or Dissertation
Format: Text
Rights:
Access: Public
License: Copyright
Holder: Lambert, Patricia
Statement: Copyright is held by the author, unless otherwise noted. All rights reserved.