Genetic analysis of the spindle checkpoint genes san-I, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans

Description:

Article on a genetic analysis of the spindle checkpoint genes san-I, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans.

Creator(s):
Creation Date: February 4, 2008
Partner(s):
UNT College of Arts and Sciences
Collection(s):
UNT Scholarly Works
Usage:
Total Uses: 111
Past 30 days: 5
Yesterday: 0
Creator (Author):
Hajeri, Vinita A.

University of North Texas

Creator (Author):
Stewart, Anil M.

University of North Texas

Creator (Author):
Moore, Landon L.

Boston University. School of Medicine

Creator (Author):
Padilla, Pamela A.

University of North Texas

Publisher Info:
Publisher Name: BioMed Central Ltd.
Place of Publication: [London, United Kingdom]
Date(s):
  • Creation: February 4, 2008
Description:

Article on a genetic analysis of the spindle checkpoint genes san-I, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans.

Degree:
Department: Biological Sciences
Note:

Abstract: Background: The spindle checkpoint delays the onset of anaphase until all sister chromatids are aligned properly at the metaphase plate. To investigate the role san-1, the MAD3 homologue, has in Caenorhabditis elegans embryos, the authors used RNA interference (RNAi) to identify genes synthetic lethal with the viable san-1 (ok1580) deletion mutant. Results: The san-1 (ok1580) animal has low penetrating phenotypes including an increased incidence of males, larvae arrest, slow growth, protruding vulva, and defects in vulva morphogenesis. The authors found that the viability of san-1 (ok1580) embryos is significantly reduced when HCP-1 (CENP-F homologue) are reduced by RNAi. Interestingly, the viability of san-1 (ok1580) embryos is not significantly reduced when the paralog of HCP-1, HCP-2, is reduced. The phenotype of san-1 (ok1580); hcp-1 (RNAi) embryos includes embryonic and larval lethality, abnormal organ development, and an increase in abnormal chromosome segregation (abberrant mitotic nuclei, anaphase bridging). Several of the san-1 (ok1580); hcp-1(RNAi) animals displayed abnormal kinetochore (detected by MPM-2) and microtubule structure. The survival of mdf-2 (RNAi); hcp-1 (RNAi) embryos but not bub-3 (RNAi); hcp-1 (RNAi) embryos was also compromised. Finally, the authors found that san-1 (ok1580) and bub-3 (RNAi), but not hcp-1 (RNAi) embryos, were sensitive to anoxia, suggesting that like SAN-1, BUB-3 has a functional role as a spindle checkpoint protein. Conclusion: Together, these data suggest that in the C. elegans embryo, HCP-1 interacts with a subset of the spindle checkpoint pathway. Furthermore, the fact that san-1 (ok1580); hcp-1 (RNAi) animals had a severe viability defect whereas in the san-1 (ok1580); hcp-2 (RNAi) and san-1 (ok1580); hcp-2 (ok1757) animals the viability defect was not as severe suggesting that hcp-1 and hcp-2 are not completely redundant.

Physical Description:

18 p.

Language(s):
Subject(s):
Keyword(s): RNA interference | embryos | genes | phenotypes
Source: Cell Division, 2008, London: BioMed Central Ltd.
Partner:
UNT College of Arts and Sciences
Collection:
UNT Scholarly Works
Identifier:
  • DOI: 10.1186/1747-1028-3-6 |
  • ARK: ark:/67531/metadc122165
Resource Type: Article
Format: Text
Rights:
Access: Public
Citation:
Publication Title: Cell Division
Volume: 3
Issue: 6
Peer Reviewed: Yes